lambda 10–2 two filter wheel system  (LAMBDA Laboratory Instruments Inc)

Journal: Scientific Reports

Article Title: Diethylcarbamazine, TRP channels and Ca 2+ signaling in cells of the Ascaris intestine

doi: 10.1038/s41598-022-25648-7

Figure Lengend Snippet: TRP channel antagonists 2-APB and SKF inhibit DEC mediated Ca 2+ signals: ( A ) Representative trace in response to 10 µM 2-APB being applied after 10 µM DEC initiates a Ca 2+ signal. Grey box represents DEC 10 µM application, bar represents 10 µM 2-APB. ( B ) Representative trace in response to 10 µM 2-APB being applied at the same time as 10 µM DEC. Grey box represents DEC 10 µM application, bar represents 10 µM 2-APB. ( C ) Total amplitudes of Fluo-3 fluorescence in response to 10 µM 2-APB (black bar), 10 µM 2-APB & 10 µM DEC (white bar), 10 µM DEC (light grey bar) and 10 mM CaCl 2 (dark grey bar). N.S. not significantly different to 2-APB (2-APB vs 2-APB + DEC P = 0.6519, t = 0.4650, df = 10, unpaired t- test). 2-APB n = 6 intestines from 3 females; 2-APB + DEC n = 6 intestines from 4 females. *Significantly different to 2-APB + DEC (2-APB + DEC vs DEC P < 0.026 t = 3.102 df = 5, paired t -test). n = 6 intestines from 4 females. † Significantly different to DEC (DEC vs CaCl 2 P < 0.0326, t = 2.931, df = 5, paired t -test). n = 6 intestines from 4 females. ( D ) Total maximal amplitudes of Fluo-3 fluorescence in response to 10 µM SKF96365 (black bar), 10 µM SKF96365 and 10 µM DEC (white bar), 10 µM DEC (light grey bar) and 10 mM CaCl 2 (dark grey bar). ***Significantly different to SKF (SKF vs SKF + DEC P = < 0.0001, t = 7.38, df = 349, paired t- test). SKF96365 n = 7 intestines from 6 females; SKF96365 + DEC n = 7 intestines from 6 females. ***Significantly different to SKF96365 + DEC (SKF96365 + DEC vs DEC P < 0.0001 t = 14.74 df = 349, paired t -test). n = 7 intestines from 6 females. † Significantly different to DEC (DEC vs CaCl 2 P < 0.0001, t = 14.82, df = 349, paired t -test). n = 7 intestines from 6 females E) Representative trace in response to 10 µM SKF96365 and 10 µM DEC. Grey box represents 10 µM SKF96365 application, bar represents 10 µM DEC. All values represented as means ± SEM.

Article Snippet: Light control was achieved using a Lambda 10–2 two-filter wheel system with a shutter controller (Lambda Instruments, Switzerland).

Techniques: Fluorescence